Chromatography and tangential flow filtration (TFF) steps are traditionally performed as separate unit operations in downstream bioprocessing. This work aims to demonstrate the capability of the UNICORN™ system control software to integrate these unit operations to eliminate intermediate hold steps thereby releasing resources and reducing overall process time. Here, the ÄKTA™ ready chromatography system was directly connected to the ÄKTA readyflux filtration system, for polishing and concentration of a target molecule. One automation method was created for control of both systems. Single-use process components were used to add flexibility to the process.
December 14, 2017
Connected Polishing and Concentration Under One Automation Method
Chromatography and tangential flow filtration (TFF) steps are traditionally performed as separate unit operations in downstream bioprocessing. This work aims to demonstrate the capability of the UNICORN™ system control software to integrate these unit operations to eliminate intermediate hold steps thereby releasing resources and reducing overall process time. Here, the ÄKTA™ ready chromatography system was directly connected to the ÄKTA readyflux filtration system, for polishing and concentration of a target molecule. One automation method was created for control of both systems. Single-use process components were used to add flexibility to the process.