This application note describes the design of circuits with low working volume, allowing for high concentration of dilute feed streams. The use of ReadyCircuit™ single-use assemblies allowed for aseptic connectivity of the filtration device. Filtration was performed using ReadyToProcess™ hollow fiber filter cartridges. The described process enabled concentration of a bovine IgG antibody from 50.0 to 0.165 L in less than 5 h.
March 23, 2017