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Human embryonic kidney 293 (HEK293) cells are widely used in production of recombinant viruses and proteins because of their stable growth, high transfection level, and ability to produce high viral titers. HEK293 cells are susceptible to infection by adenovirus, one of the most frequently used viral vectors in the development of therapeutic vaccines and vaccines against infectious diseases. This application note demonstrates growth of HEK293 cells in CDM4HEK293 medium in both shake flask and bioreactor cultures. Adenovirus productivity was evaluated in shake flask cultures. Performance in CDM4HEK293 was compared with performance in a reference medium.
October 19, 2017
Evaluation of HEK293 Cell Growth and Adenovirus Productivity in HyClone™ CDM4HEK293 Medium
Human embryonic kidney 293 (HEK293) cells are widely used in production of recombinant viruses and proteins because of their stable growth, high transfection level, and ability to produce high viral titers. HEK293 cells are susceptible to infection by adenovirus, one of the most frequently used viral vectors in the development of therapeutic vaccines and vaccines against infectious diseases. This application note demonstrates growth of HEK293 cells in CDM4HEK293 medium in both shake flask and bioreactor cultures. Adenovirus productivity was evaluated in shake flask cultures. Performance in CDM4HEK293 was compared with performance in a reference medium.