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This article summarizes the results of Escherichia coli growth performance for plasmid production in a 5 L glass fermentor, which was scaled up to 80 and 160 L scale in Xcellerex™ XDR-200 MO single-use fermentors. We used an E. coli DH5α strain which produced a pLP2 plasmid of 4180 bp in the study. Performance of peak E. coli growth measured as optical density at 600 nm (OD600), plasmid titer, and percentage of produced supercoiled (SC) plasmid DNA was comparable at all culture volumes tested.