The use of disposable technologies in downstream processing has increased significantly over the last years, as they reduce the need for time-consuming cleaning and cleaning validation operations as well as eliminate startup procedures such as column packing. The challenge, however, of using disposable technologies relates to the often higher consumables and operating cost as compared with stainless steel solutions. As projects are scaling up to manufacturing scale, it becomes attractive to cycle equipment for specific campaigns for full capture of process economy gains. This customer case study evaluates process performance and economy when reusing a prepacked ReadyToProcess™ 32 L (450/200) chromatography column over several cycles in a multiproduct facility. The results indicate that cycling and reusing ReadyToProcess 32 L columns give consistent performance and enables significant process economic benefits.