Screening of chromatographic conditions can be done in several ways, such as 96-well plates, mini-columns, and tips. It is important that the screening can give an indication of the optimal conditions for binding and/or elution in a chromatographic column run. The aim of this study was to investigate conditions for reduction of host cell proteins (HCP) on a strong cation exchanger, Capto™ S ImpAct. Three different screening techniques using PreDictor™ plates are compared and discussed. Selected conditions were applied in column step elution experiments and antibody yield, pool volume and reduction of HCP was compared.
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