A majority of cell lines commonly used for viral vaccine production are anchorage-dependent and require expansion on solid surfaces. Here, we describe different approaches for the cultivation of Vero cells using Cytodex and Cytodex Gamma microcarriers in serum-free cultivation medium using spinners, WAVE Bioreactor™ 20/50, ReadyToProcess WAVE™ 25, and Xcellerex™ XDR-10 single-use bioreactor systems. Cells in each WAVE Bioreactor system were infected with influenza virus and the concentration of infectious virus was determined by 50% tissue culture infective dose measurement. The results show virus titer activity was comparable for Cytodex and Cytodex Gamma and there was no observed difference in distribution or cell growth between systems.