Search results for " recombinant"
Article
Platform Technologies Improve Protein Expression
“In the rapidly growing market of recombinant proteins and antibodies, our STEP technology provides a tool to complete preclinical phases at higher speed with reduced costs and with a higher success r…
Poster
Scale-up of CHO fed-batch cultures in HyClone™ ActiPro™ medium supplemented with Cell Boost™ 7a and 7b
Comparing cell growth and recombinant protein production of multiple Chinese hamster ovary (CHO) cell clones when cultured in HyClone ActiPro basal medium and Cell Boost 7a and 7b feeding supplement…
Article
Antibody Purification Handbook
The use of recombinant technology opens up the potential to create an infinite number of combinations between immunoglobulins, immunoglobulin fragments, tags and selected proteins, further manipulatin…
Article
Automated harvesting and 2-step purification of unclarified mammalian cell-culture broths containing antibodies
… followed by in-line tandem affinity-capture, pH neutralization and size-exclusion chromatography of recombinant antibodies expressed transiently in mammalian human embryonic kidney 293T-cells at the…
Article
Optimization of Protein Expression in Escherichia Coli
There are many parameters that affect cell growth and recombinant protein expression, such as choice of medium, carbon source, temperature, pH, aeration, inducer concentrations, and length of inductio…
Article
USP Publishes Monoclonal Antibody Guidelines
In 2012, USP started working on a clearly defined set of quality expectations for recombinant therapeutic mAbs, United States Pharmacopeia (USP) General Chapter “Analytical Procedures for Recombinant…
Article
Comparing Protein A Resins for Monoclonal Antibody Purification
The gene for Protein A has been cloned and expressed in Escherichia coli (6, 7) allowing for the production of large quantities of recombinant Protein A.
Although recombinant Protein A is widely u…
Article
Bioprocessing Advances in Vaccine Manufacture
…ity (to be operated by G-Con, developer of the modular units being used) in Texas for manufacture of recombinant tobacco plant-expressed influenza vaccines, with a projected final scale capacity of 1…
Article
HT Multi-Product Liquid Chromatography for Characterization of Monoclonal Antibodies
Capillary electrophoresis sodium dodecyl sulfate nongel sieving analysis of a therapeutic recombinant monoclonal antibody: a biotechnology perspective. Anal Chem. 1999;71:2390–97.
20. Ma S. Analys…
Article
Quality by design for biotechnology products—part 1
These strategies include engineering the primary sequence to incorporate chemical and post-translational modifications for improved stability; recombinant and chemical fusion approaches for improved h…