Search results for "column"
Article
Using Digital Twins to Model Process Chromatography
Once a molecule enters the pore system, its behavior follows the same mechanisms in an adsorber bead packed in a filter plate and a production column. To allow for a prediction of the complete process…
Article
Setting Up Bioprocessing Systems for Digital Transformation
Hahn points out that, for instance, pre-packed single-use chromatography columns of the same type may differ in terms of column packing and resin lot capacity. “In practical application, these differe…
Article
Purify App: Finding What You Need for Protein Purification
…stem to match your application?
Visit the NEW purify app to:
• select resins, prepacked columns, and empty columns
• configure and visualize ÄKTA systems in 3D
• find accessori…
Article
New HiScale™ 10/40 for Scale-Down Studies
HiScale 10/40 column is especially suitable for scale-down studies used in, for example, viral clearance evaluation in downstream processing. The small inner diameter (10 mm) and flexible adapters min…
Article
Chromatography Scale-Up: Don’t Get Tied Down by Bed Height
But aiming for a constant bed height throughout scales has a limitation—you need an exact chromatography column size that fits the purpose.
Finding the right column size can be tricky. The articl…
Article
Going Small to Achieve Success on the Commercial Scale
A scaled-down chromatography process, for instance, is designed considering the residence time, flow rate, and column bed height of the large-scale process. Additional parameters that are considered i…
Article
A Closer Look at Affinity Ligands
The matrix is typically in the form of small 50–150 micron beads that are highly porous to provide a large surface area, maximizing antibody binding potential for higher yields from fixed-volume colum…
Article
Top Tips for Successful Development of Antibody Chromatography Processes
There are also small, miniature columns that can be utilized in the same way. So that will speed up the experiments that you're running, but also utilize the design of experiment, where you want to ge…
Article
Moving PAT from Concept to Reality
In column chromatography, for instance, analysis cannot be performed until after the column, preventing any in-process monitoring. The diversity of downstream processes is also a consideration. “Not o…
Article
How to Determine Dynamic Binding Capacity (DBC) of Chromatography Resins
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In protein purification, dynamic binding capacity (DBC) of a chromatography column describes the maximum amount of target protein that you can load onto your co…