Search results for " fed-batch"
Poster
Development of DoE based fed-batch strategies for high-producing CHO cell cultures
To mimic more closely the dynamics of a fed-batch culture, we developed a dynamic feeding strategy responsive to the actual nutrient needs of a mAb-producing recombinant Chinese hamster ovary (CHO) ce…
Poster
Effects of feeding strategy on CHO cell performance in fed-batch cultures
This work demonstrates the development of a fed-batch process for a Chinese hamster ovary (CHO) cell line producing a monoclonal antibody (mAb). The results emphasize the importance of an appropriate …
Article
Fed-batch supplements to boost cell culture performance
Cell growth and MAb production in the fed-batch cultures were compared with batch cultures. The results demonstrate increased viable cell yields and productivity in the fed-batch cultures.
Downloa…
Poster
Repurposing Fed-Batch Media and Feeds for Highly Productive CHO Cell Perfusion Processes
The authors developed a method for identifying first-generation perfusion culture media based on existing fed-batch media and feeds. Here, they show that they can obtain culture media that successfull…
Article
Perfusion N-1 – A Way to Improve the Fed-Batch Production Process
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In this article, we show how the conventional N-1 seed train step was replaced by a high-density perfusion and how the production bioreactor can be seeded at a 10-fol…
Article
Intensifying your fed-batch process using cryobags
We will show that high-density cryobags can be used to seed a bioreactor at a 500-fold higher seed density, reducing the total process time by up to 9 days.
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Article
Simple Fed-Batch Process Development for a Knockout Cell Line
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This workflow improved titers by 500% compared with Chinese hamster ovary cell culture in basal medium.
Poster
Scale-up of CHO fed-batch cultures in HyClone™ ActiPro™ medium supplemented with Cell Boost™ 7a and 7b
Comparing cell growth and recombinant protein production of multiple Chinese hamster ovary (CHO) cell clones when cultured in HyClone ActiPro basal medium and Cell Boost 7a and 7b feeding supplement…
Article
Perfusion in the 21st Century
Batch processing, in which all raw materials are introduced into the bioreactor at the beginning of the cell-culture process, have largely been replaced with fed-batch systems, in which additional nut…
Article
Unifying Continuous Biomanufacturing Operations
Lawrence Yu, PhD, deputy director in the Office of Pharmaceutical Quality at the Center for Drug Evaluation and Research (CDER), said in a 2016 FDA blog post that processes that take a month with fed-…