Search results for "antibodies" in Articles / App Notes
Article
Selection Guide: Columns and Resins for Antibody Purification and Immunoprecipitation
Polyclonal antibodies, monoclonal antibodies (mAbs), and antibody fragments are usually purified by affinity chromatography. Resins containing an immobilized ligand (e.g., protein A, protein G, or p…
Article
Impact of Media Components on CQAs of Monoclonal Antibodies
It is effective in producing antibodies with oligomannose-type glycans from CHO cells (2, 10). Gallic acid and its derivatives efficiently inhibit fucosylation in the presence of 10-15 mM Mn2+. Poloxa…
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Automated harvesting and 2-step purification of unclarified mammalian cell-culture broths containing antibodies
…in-line tandem affinity-capture, pH neutralization and size-exclusion chromatography of recombinant antibodies expressed transiently in mammalian human embryonic kidney 293T-cells at the 1-L scale. T…
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Selecting Chromatography Resins for Bispecific Antibody Purification
Their purification can be more challenging than traditional monoclonal antibodies (mAbs), however, due to the increased level of product-related impurities with high similarity to the desired bsAbs. A…
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A Platform Approach to Purification of Antibody Fragments
As compared with full length antibodies, their fragments have unique properties that make them favorable for certain therapeutic conditions. However, due to their high molecular diversity, the use of …
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Resolving a bottleneck in screening and characterization of recombinant antibody fragments using Biacore 4000
Techniques to screen and characterize large numbers of antibodies and provide confident selection of the best candidates for development are therefore essential. Biacore 4000 equipped with Biacore 400…
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Exploring the world of antibodies: mAbs, Fabs, dAbs, and bsAbs
There is a vast array of antibody variants, including multispecific (msAb), bispecific antibodies (bsAb), antibody fragments (Fab, dAb, scFv), and antibody-drug conjugates (ADC). But with this…
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Pushing the Barriers in Next-Gen Antibody Development
Continued development of new and next-generation antibodies is spurred by the increasing demand for more effective and custom-tailored biologic therapies. In particular, bispecific antibodi…
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Capture of Bispecific Antibodies and Removal of Product-Related Impurities
Many of these variants, especially asymmetric bispecific antibodies, are prone to aggregation or to forming product-related impurities such as homodimers and half antibodies during cell culture. The s…
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Chromatography innovations for the diverse antibody pipeline
To support development of new therapeutic possibilities, manufacturing and process development technologies must also evolve. For antibody variants, platform purification protocols for conve…