Search results for " separation" in Articles / App Notes
Article
In silico optimization of AAV full/empty particle separation with AIEX
We present a guiding workflow showcasing in silico process optimization of full and empty AAV5 particle separation on strong AEX resins, such as Capto™ Q ImpRes or Capto™ Q with GoSilico™ chromatograp…
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Alternative solutions to separate AAV full and empty capsids using anion exchange
Here we present high-performance anion exchange (AEX) chromatography separation for several AAV serotypes using step-elution mode. Optimized protocols and conditions for both resin and membrane format…
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How to optimize AAV capsid separation
In this free, interactive course you’ll learn how to tackle one of the key challenges in Adeno-associated virus (AAV) manufacturing–separating full and empty capsids in the polishing step. You’ll …
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Buffer optimization for separation of bsAb in a capture step using MabSelect™ VL resin
Protein L resins bind to kappa light chain and are often used to purify antibody fragments such as dAbs and Fabs — but they can also be used to remove product-related impurities from bispecif…
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Tools and Solutions for Separation of Charged mAb Variants
A biosimilar is an almost identical version of an originator product. To attain regulatory approval, a comparable quality to the reference product in terms of efficacy, purity, and safety should be …
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SEC in the Modern Downstream Purification Process
…ng purified for further processing or formulation and storage without having an effect on the actual separation. This lack of chemical interaction allows SEC to have a significant advantage over othe…
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Process Chromatography: Continuous Optimization
Drug manufacturers are always seeking ways to improve the efficiency and productivity of chromatographic separations. Suppliers of chromatography resins are developing new resin chemistries and bindin…
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N-Glycan Analysis of Biotherapeutic Proteins
N-glycan labeling
Released N-glycans are frequently labeled with a fluorophore to enhance glycan separation and detection (34). Commonly used labels include 2-aminobenzamide (2-AB) and 2-aminob…
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HT Multi-Product Liquid Chromatography for Characterization of Monoclonal Antibodies
…eity of proteins, yet despite good resolving power and robustness, ionic strength-based ion exchange separations are product-specific and time-consuming to develop. Some improvements have been made t…
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Process Chromatography Selection for Downstream Processing Applications
Selecting the appropriate separation technology plays a big role in maximizing productivity in any downstream processing application. Some factors companies may consider when selecting a separation te…