Search results for " virus"
Article
The Development and Application of a Monoclonal Antibody Purification Platform
The eluate was then held at this low pH to inactivate viruses, and then adjusted to increase the pH before the cation exchange chromatography step.
After cation exchange, diafiltration was perf…
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Single-Use Technologies Prove Effective for Viral Vector Process Development
Notably for the manufacture of vectors based on adeno-associated virus (AAV), one of the most common vector types for gene therapy delivery and viral-vector vaccines, recent data have shown that stain…
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Vaccine Development Faces Urgency and Challenges
By Jill Wechsler
The Ebola outbreak in 2015 and the current stampede to develop a new vaccine to combat the Zika virus illustrate the increasingly important role of vaccines in advancing public he…
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Report from the Eighth International Plasma Product Biotechnology Meeting Melia Salinas, Lanzarote, Spain
…says and possible also implementing heat treatment to the relevant plasma products to inactivate the virus. In the Pathogen Safety session an illustrative presentation was given by Jun Adan-Kubo from…
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A Look at the Affinity Chromatography Landscape
Another option is the use of monolithic, convective chromatography supports, which are widely used for the purification of macromolecules such as viruses, virus-like particles, plasmid DNA, and cells.…
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Unifying Continuous Biomanufacturing Operations
He explains that stable continuous virus production is not possible if the cell and the product (virus) are propagated in the same vessel because viruses are lytic. “For continuous virus production, m…
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SEC in the Modern Downstream Purification Process
…size-exclusion chromatography (SEC) has played a significant role in the purifiĀcation of proteins, viruses, enzymes, nucleic acids, and other biomolecules. While SEC may have mostly been abandoned …
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Advances in Sterilization Technologies for Overcoming Viral-Vector Manufacturing Challenges
Today, however, the variable nature of viruses (DNA/RNA, enveloped/non-enveloped, size range from 20 nm to 400 nm) and their associated properties (e.g., chemical, physical, and thermal stability) rem…
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Filtration methods to overcome new challenges in viral safety
41 and ICH Q5A, and outlines the detailed mechanisms governing effective virus filtration, demonstrating how both specific methods and advanced filter design can overcome these challenges.
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Maximum Output Starts with Optimized Upstream Processing
…ell clones, using technologies to achieve high cell densities and perfusion cultures, and optimizing virus recovery. In addition, Lipinski notes that the use of high-efficacy viral vector platforms t…