Search results for " bioreactor" in Articles / App Notes
Article
Best Practices for Selecting a Top-Quality Cell Line
Before scaling up to bioreactors, the productivity level for monoclonal antibodies (mAbs) should be 2–7 g/L (fed-batch culture in shake flask) or greater, observes Selexis CEO Igor Fisch. Once scaled …
Article
Ensuring Viral Safety of Viral Vaccines and Vectors
Extraneous vesiviruses, however, have recently appeared in bioreactors (2–5), and porcine circovirus type 1 (PCV-1) contamination of oral rotavirus vaccines was first reported by a metagenomics analys…
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Optimizing Late-Stage and Commercial Cell-Culture Processes
This resulted from the introduction of parental cell lines better suited to modern fed-batch processes and the development of better bioreactor processes, including control strategies for pH, carbon d…
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Challenges in Cell Harvesting Prompt Enhancements
Biomanufacturers have been shifting from large stainless-steel bioreactors in the thousands-of-liters range to smaller, disposable bioreactors (2).
The need to address this bottlenecking issue …
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Designing a Biomanufacturing Facility Incorporating Single-Use Technologies
For example, one can consider 2 x 2000-L bioreactors running a typical 14-day incubation period staggered a week apart, which equates to one batch produced each week. A typical batch at 2 g/L with a 7…
Article
A Risk-Based Genetic Characterization Strategy for Recombinant CHO Cell Lines Used for Clinical and Commercial Applications
Generation 0 (G0) represents the generation of a master cell bank, G30 represents the generation of cells harvested from a 5000-L bioreactor, and G60 represents the limit for in vitro cell age designe…
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Implementation of Raw Material Control Strategies in the Manufacture of Single-Use Bioprocessing Containers
Disposable bioprocessing containers, such as those used for fluid storage or as bioreactor bags, are usually made from multilayer films. Typical resins used in multilayer films include, but are not li…
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Improving PAT for Biologics
“We are excited about the use of Raman and NIR probes for biopharmaceutical manufacturing because these probes have the ability to provide real-time information about the process and product in the bi…
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Stirring up the fermentation game
… technology by biomanufacturers using microbial systems has been inhibited by the lack of single-use bioreactor systems capable of accommodating the unique requirements of microbial cultures. Bioreac…
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Overview of a scale-up of a cell-based influenza virus production process using ReadyToProcess equipment
The cells in the 10 L cultures were grown on Cytodex 1 microcarriers (3 g/L) in the WAVE Bioreactor 20/50 system. The cells were grown to a cell concentration of approximately 3 × 106 cells/mL. The ce…