Search results for "buffer"
Article
Rapid Cycling Protein A-Based Fiber Chromatography to Address Emerging Downstream Bottlenecks
Fibro PrismA is a new protein A fiber chromatography format developed to complement traditional resin-based chromatography. The protein A fiber matrix has an open pore structure in which mass transf…
Article
Optimized Process Efficiency in Upstream Manufacturing
Process efficiency is a key goal for biopharmaceutical production. Click here to learn how faster cell-line development, real-time analytics, and other approaches can be used to create a flexi…
Article
Tools and Processes for Mature and Emerging Therapies
…ed options for upstream and downstream systems, and introduced equipment, supplies, columns, media, buffers, single-use systems, and ancillary equipment to promote process capabilities and reliabilit…
Article
A Look at the Affinity Chromatography Landscape
He points to engineered antibody affinity resins and mild-pH elution Protein A resins that enable elution of mAbs without the use of traditional, low-pH elution buffers as important examples.
“…
Article
A Plastic Pipeline for Commercial Bioprocessing?
More than 80% of the respondents use tubing, disposable filter cartridges, empty bags, connectors and clamps, and buffer containers. More than 75% use filled media bags, preassembled tubing sets, dept…
Article
Putting Viral Clearance Capabilities to the Test
“For example, if one step inactivates virus due to low pH, then a second step that uses a low pH buffer may not also be included, since virus that escapes inactivation by one low pH step is likely to …
Article
Leveraging Data for Better Biopharmaceutical Process Control
For example, chromatography data can provide a fingerprint of the product at a specific point in the process so that changes in peak shape can be used to better understand the underlying buffer prepar…
Article
Flow Accuracy and Gradient Performance of ÄKTA pilot 600 Chromatography System
Within the buffer B gradient range (%B) from 1%B to 99%B at a system flow rate of 5–600 mL/min, gradient accuracy was found to be ±1%B.
Explore the next generation ÄKTA, ÄKTA pilot 600
Article
Preclinical Evaluation of Product Related Impurities and Variants
The refolded samples were subsequently buffer exchanged and subjected to the downstream purification.
The choice of the purification platform is crucial for this study. The platform should be …
Article
Asked and Answered: Your Questions on Ion-Exchange Chromatography
The questions are organized in by chief concern, and include buffers, experimental design, LC system and configuration, resin and column selection, specific applications, as well as troubleshooting an…