Search results for "buffer"
Article
Detecting Protein Aggregates and Evaluating their Immunogenicity
Higher concentrations of a protein formulation can increase the probability of aggregation, as well as changes in solution pH, temperature, buffer choice, agitation, and storage conditions. “Although …
Article
SEC in the Modern Downstream Purification Process
SEC can also be used as a fast, simple step for buffer exchange. Small molecules such as salts and unconjugated labels or payloads are easily separated from larger species in desalting and buffer-exch…
Article
Analyzing Proteins Using SEC, MALS, and UHPLC
The range of columns and buffers available for use in SEC, however, means that there are always improvements to be made in method development, according to Pothecary. “Alternative columns, different …
Article
Under pressure: exploring root causes of leaks in single-use mixers
More minor issues, like buffer leaks during a mixing step, are less costly, but they still have an impact – increasing the time, material, and consumable use for that step.
Here we’ll take a look …
Article
Advances in Sterilization Technologies for Overcoming Viral-Vector Manufacturing Challenges
Finally, Langhauser highlights the increased availability today of GMP-compliant small tangential-flow filtration (TFF) systems with peristaltic pumps for performing buffer exchange at extremely low v…
Article
FAQs about Cytiva™ Protein Select™ technology
The protocol does not require the use of a protease, and the same buffer can be used for equilibration, binding, wash, and elution.
This FAQ page provides answers to a range of questions covering …
Webcast
Cytiva Protein Select Technology Live Webinar
The protocol does not require the use of a protease, and the same buffer can be used for equilibration, binding, wash, and elution.
Article
Webinar about a new affinity chromatography technology for recombinant proteins
The protocol does not require the use of a protease, and the same buffer can be used for equilibration, binding, wash, and elution.
Watch this webinar to learn more about this new affinity chrom…
Article
On-demand webinar: Balancing innovation, speed, and robustness to improve efficiency in upstream process development
Did you miss our webinar on accelerating biopharma development cycles? Watch it now, on demand! We'll cover strategies like cell line development, media development, and raw material characte…
Webcast
Balancing innovation, speed, and robustness to improve efficiency in upstream process development.
Date: April 26, 2023 2:00pm CEST(8:00am EDT)
Register today