Search results for " techniques" in Articles / App Notes
Article
Fundamentals of hydrophobic interaction chromatography
Hydrophobic interaction chromatography (HIC) is a versatile technique that can be used for capture, intermediate purification, or polishing steps.
This technique separates proteins accor…
Article
Good Modeling Practice
Standard model calibration techniques may result in unreasonable correlations and unrealistic physical parameter estimates. But how can we mitigate this risk, improve the model’s quality, and reduce t…
Article
New hydrophobic interaction chromatography (HIC) handbook
Hydrophobic interaction chromatography (HIC) separates biomolecules under mild conditions according to differences in molecular hydrophobicity. HIC is often used as a next step to purify prote…
Article
Enhancing Resins Addresses Purification Concerns
“There are several purification techniques that are in use, such as precipitation, tangential flow filtration, or chromatographic separations. However, precipitation or size-exclusion chromatography (…
Article
Assessing Manufacturing Process Robustness
Criticality assignment (Step 2)
Upon identifying the risk factors through cause-and-effect techniques, a risk-rating decision tree (Figure 3) is used to analyze the process upon risk asse…
Article
Setting Up Bioprocessing Systems for Digital Transformation
This is an area where AI, in conjunction with online and rapid offline PAT techniques and digital twinning, will allow real-time process, Kuerten explains.
Legacy transformation
In terms of a …
Article
The Importance of Process Intensification and PAT for Achieving Real-Time Release
Sharing of best practices and potential new analytical techniques at early stages of development can be very powerful.
All stakeholders in the industr…
Article
Typical Purification Protocols to Obtain the Right Purity and Yield
Our free digital guide helps you effectively combine the main chromatography techniques to obtain a powerful purification protocol.
Article
The Need for Advanced Process Modeling for New Therapeutic Biologics
Sinclair notes that there are specific challenges to some of the newer personalized therapies and gives the following examples of modeling techniques that can be broken into several areas:
…
Article
Biosimilar Quality Requirements
For higher-order structural analysis, various biophysical techniques are mainly employed, according to a paper published by M. DiPaola and I. Javeri (5).
Mass spectrometry has been known as a key …