Search results for " cell-culture"
Article
Best Practices for Selecting a Top-Quality Cell Line
Factors such as cell growth and metabolism, as well as product quality attributes like glycosylation distribution, are closely related to the culture medium, and thus many cell-culture media component…
Article
Fifteen Years of Progress: Biopharmaceutical Industry Survey Results
In contrast, upstream concerns, “optimizing cell-culture systems,” was number one in 2003.
Exciting technologies have come and gone over the past 15 years. For example, back in 2003, transgenic…
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N-Glycan Analysis of Biotherapeutic Proteins
High mannose glycans represent an immature glycan structure, and may be the result of suboptimal CHO cell-culture conditions (20). The presence of high-mannose glycans results in increased clearance m…
Article
Being Thorough When Transferring Technology
…r if the scalability from the small-scale to large-scale is unknown; for example, in the scale-up of cell-culture production bioreactors. It is not uncommon to see slight differences not only in how …
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Critical Quality Attributes Challenge Biologics Development
With the advancement of knowledge in analytical sciences, protein engineering, biology, and cell-culture process, it is now possible to assess quality attributes more objectively than before. The awar…
Article
Cost Considerations Drive Lean Technology in Biopharmaceutical Manufacturing
Alternatively, upstream process scale-up can make use of perfusion reactors, which use a constant supply of cell-culture media while removing unwanted byproducts throughout a prolonged production run …
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Process Chromatography Selection for Downstream Processing Applications
If onsite systems cannot meet the needs for buffer and cell-culture media preparation, these can be bought from sub-suppliers rather than prepared onsite.
As facility utilization continues to be t…
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Generating a Fully Processed Antibody
This use of multiple selectable markers in a cell line can complicate cell-culture processes, adding an increased burden on the cell-line machinery to produce multiple extra proteins. In addition, pro…
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A Q&A on Bioprocessing Trends and Technologies at INTERPHEX 2016
Did you see any trends in the consumables segment?
The quality and supply of cell-culture media, buffers, and other process liquids was also a popular topic. Demand for custom formulations and pac…
Article
Antibody Production in Microbial Hosts
The authors review the status of expression of antibodies in microbial hosts and present the recent advances in the production of aglycosylated antibodies in bacteria.
By Anurag S. Rathore, Jyot…