Search results for " concentration"
Article
Challenges of Protein Aggregation During Purification
“Aggregate formation is generally dependent on the solution conditions (conductivity and pH), protein concentration, and temperature. In addition, aggregates can be soluble when relatively small and i…
Article
Defining Critical Quality Attributes for Monoclonal Antibody Therapeutic Products
Figure 8A illustrates the levels of site-specific methionine oxidation measured by LC-MS peptide map in the presence of increasing concentrations of an oxidizing agent. This study found that although …
Article
Elucidating Biosimilars Characterization
…hose for classical medicinal-chemistry small-molecule products and are based on a comparison of peak concentration, time-to-peak concentration, area under the concentration-time curve; however, depen…
Article
The Development and Application of a Monoclonal Antibody Purification Platform
After anion exchange, the flow-through fraction containing the antibody was passed through a nanofiltration unit to remove potential viruses and then formulated in buffer in a final concentration and …
Article
Address the complexity of protein characterization
This presentation includes some examples on the use of Biacore™ surface plasmon resonance (SPR) systems for active concentration measurement, target binding, and describe how you can use these assays …
Article
Buffer optimization for separation of bsAb in a capture step using MabSelect™ VL resin
This video presents a study of the effects of different buffer substances, mono-, di-, and tri-carboxylic acids, and concentrations on elution pH, resolution between hetero- and homodimers for bsAb’s,…
Article
From Bottlenecks to Breakthroughs: Mastering Buffer Management
Unlock innovation in manufacturing by optimizing your buffer production.
Agenda
- Options to intensify buffer preparation from using pre-made buffers or buffer concentrates to impl…
Article
Evaluating the Use of Continuous Chromatography
In Cytiva’s case, its system can adjust accordingly to changes in feed concentration, for example, to optimize processing of the perfusion output. Also, pre-packed columns have provided greater consis…
Article
Exosome isolation by tangential flow filtration and size exclusion chromatography
The workflows we describe are suitable for scale-up and combine tangential flow filtration (TFF) for the concentration of exosomes followed by gentle size exclusion chromatography (SEC) with our next-…
Article
Report from the 6th International HTPD Conference
Several analytical assays for AAV require high concentration of viral particles and therefore requires large volumes of starting material, which is not always available during process development.
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