Search results for " process"
Article
Performance of Single-Use Xcellerex™ XDR‑500 MO and XDR‑50 MO Stirred-Tank Fermentor Systems
coli was used as the model process. Fermentations were performed as fed‑batch processes, with glycerol feed and a two‑stage induction strategy. The presented data was collected as foundational sin…
Article
Start With the End in Mind: GMP Tech Transfer
And, learn how to apply their approach to your process.
Poster
Development of DoE based fed-batch strategies for high-producing CHO cell cultures
Typical standard platform processes rely on fixed-volume bolus feeding of concentrated supplements at regular intervals. However, such static approaches might result in over- or underfeeding. To mimic…
Article
Improvements in Protein A Chromatography
Process Development Forum speaks about Protein A chromatography with Glen Bolton, Scientific Director at Amgen. Bolton recently published a paper “Role of more than 40 years of improvement in Protei…
Article
Accelerated flavivirus vaccine production with modern tools and solutions
For downstream processing, modern chromatography resins offer high selectivity and excellent pressure-flow properties for high productivity inmanufacturing-scale purifications. This white paper includ…
Poster
Development of a chemically defined medium for virus vaccine production in a duck suspension cell line
The primary goal of this project was to develop a chemically defined medium that could be used in a monophasic process while supporting the growth and production of a broad spectrum of different virus…
Poster
Effects of feeding strategy on CHO cell performance in fed-batch cultures
This work demonstrates the development of a fed-batch process for a Chinese hamster ovary (CHO) cell line producing a monoclonal antibody (mAb). The results emphasize the importance of an appropriate …
Article
Efficient Purification of the Pertussis Antigens Toxin, Filamentous Haemagglutinin, and Pertactin in Chromatography Workflows
The described method resulted in higher purity and yield than what can be expected from a purification process based on salt precipitations and sucrose gradient centrifugation.
Download applicatio…
Article
Development of column packing methods based on pressure flow measurements
Scale-up of a chromatography process might appear as simply increasing the column diameter to accommodate for the higher quantity of material to be processed, while maintaining the same bed height …
Article
Fast Turnaround with Automated Column Packing
The process can consume essential time and resources. Risking poor performance with a suboptimally packed bed is not an option. Packing success is, therefore, key to ensure an efficient purification p…