Search results for " glycosylation"
Article
Leveraging Computational Models of Glycosylation for Biopharma QA
Glycosylation is also widely acknowledged as a major source of therapeutic protein heterogeneity (3), which is highlighted by work that found considerable glycosylation variation among different comme…
Article
Implications of Cell Culture Conditions on Protein Glycosylation
Richard Easton
This article reviews the implications of cell-culture conditions on biologic product quality, focusing on glycosylation and analytical techniques for its accurate assessment. Glyco…
Article
Analysis of Glycosylation in Biosimilars
The most notable of these is glycosylation, where carbohydrate residues are attached to the protein chain.
The variety of ways in which the monosaccharides in a glycan can be linked leads to a lar…
Article
N-Glycan Analysis of Biotherapeutic Proteins
By Aled Jones
Protein glycosylation, the addition of single sugars or oligosaccharide chains (glycans) to a peptide backbone, is a common post-translational modification (PTM) that imparts various…
Article
Antibody Production in Microbial Hosts
Since the advent of an era of antibody-based therapeutics, the major focus of the industry has been on the generation of antibodies with glycosylation patterns similar to the naturally occurring immun…
Article
Elucidating Biosimilars Characterization
Proteins produced in different mammalian cell lines, or under different environmental conditions, may be expressed with subtle but important differences with respect to characteristics such as glycosy…
Article
Impact of Media Components on CQAs of Monoclonal Antibodies
The cell-culture process and media are known to have a significant effect on the expression and stability of therapeutic products as well as their critical quality attributes (CQAs) including glycosyl…
Article
QbD and PAT in Upstream and Downstream Processing
Protein glycosylation, for example, is generally determined during cell culture and minimally altered in downstream unit operations (at least for uncharged glycosylation species). Using this definitio…
Article
Fermentation for the Future
Microbial expression is often preferred over cell culture for the production of smaller peptides and proteins that do not require glycosylation because the desired substances may be produced in much s…
Article
Best Practices for Selecting a Top-Quality Cell Line
Special attention in this case should be paid to critical quality attributes such as glycosylation, which can add a variable to clonal selection and process development and can lead to a scenario wher…