Search results for " resin" in Articles / App Notes
Article
Increasing Productivity in Hydrophobic Interaction Chromatography (HIC) Using Capto™ Resins
An application example is also presented in which aggregate removal results are compared using Capto™ Butyl ImpRes and Butyl Sepharose High Performance resins.
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Article
Cleaning Protein A Resins with 1 M NaOH
If not removed, these impurities can build up on the protein A resin and start to leach into the process material in subsequent cycles. The presence of cell culture nutrients in the harvest feed loade…
Article
Capacity and Performance of a Next Generation Protein A Chromatography Resin
The resin exhibits improved capacity over its predecessor MabSelect SuRe™ products, while maintaining purification performance. This application note demonstrates the binding capacity and purification…
Article
Efficient Cleaning-In-Place Methods for Protein-Based Antibody Affinity Chromatography Resins
Various cleaning strategies and the impact of different cleaning agents and concentrations on the resin lifetime are presented. As the use of sodium hydroxide (NaOH) is considered standard in the biop…
Article
Guide for selecting affinity resins for mAbs, bsAbs, and antibody fragments
Selecting an affinity resin for the diverse range of antibodies can be overwhelming. But don’t worry, in this selection guide you will find the antibody affinity resin to achieve your required purity …
Article
Alkaline stability of Fc and VH3 binding in protein A resins
We evaluated NaOH incubation and the effect of performed CIP cycles for two established protein A resins on the market.
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Article
Selection Guide: Columns and Resins for Antibody Purification and Immunoprecipitation
Download the latest version of the selection guide Columns and Resins for Antibody Purification and Immunoprecipitation to help you select the most suitable affinity column for purifying your antibody…
Article
Maximize resin usage with continuous bioprocessing
Periodic counter-current chromatography (PCC) is a multi-step approach to maximize the chromatography resin capacity utilization and minimize process time. The technology employs three or more chromat…
Article
Buffer optimization for separation of bsAb in a capture step using MabSelect™ VL resin
…r bsAb’s, and removal of light chain from mAbs during elution when using MabSelect™ VL protein L resin. The video includes results of these experiments and a discussion of how buffer screening enable…
Article
Process development using a nonaffinity method vs Cytiva™ Protein Select™ resin
The first method is using nonaffinity chromatography steps (AIEX + heat treatment + HIC), while the second method is using the novel affinity Cytiva™ Protein Select™ resin for the capture step, follow…