Search results for " microcarrier" in Articles / App Notes
Article
Production of Recombinant Adeno-associated Virus Vectors Using Microcarrier Culture of HEK293T Cells
As of March 2020, more than 4310 gene therapy clinical trials have been performed worldwide, with only 518 of them in Phase III and Phase IV. As the gene therapy projects enter clinical Phase III or g…
Article
Downstream Processing for Cell-Based Therapies
Significant progress has been made in recent years with human mesenchymal stem cells (hMSCs) cultured on microcarriers now reaching densities of >1x106 cells mL-1 (4) and human pluripotent stem cells …
Article
Overview of a scale-up of a cell-based influenza virus production process using ReadyToProcess equipment
The cells in the 10 L cultures were grown on Cytodex 1 microcarriers (3 g/L) in the WAVE Bioreactor 20/50 system. The cells were grown to a cell concentration of approximately 3 × 106 cells/mL. The ce…
Article
Adherent Cell Culture in Biopharmaceutical Applications: The Cell-Detachment Challenge
Degree of cell adhesion
Vigorous cell-culture processes, such as culture on microcarriers, require strong cell adhesion that needs be matched by the harshness of the detaching step. On the other h…
Article
Validation of the production of influenza virus in ReadyToProcess WAVE™ 25 bioreactor system
To reduce the need for microcarrier preparation prior to use, presterilized and ready-to-use Cytodex Gamma microcarriers was evaluated and compared with Cytodex microcarriers. The results show compara…
Article
Single-Use Technologies Prove Effective for Viral Vector Process Development
Use of microcarriers that support adherent cell lines is also being explored as a means for scaling adherent processes in suspension in stirred-tank bioreactors.
Flatware is often used in the seed…
Article
Scalable ADCF Virus Production Using HyClone™ VaccineXpress Medium
Small-scale microcarrier cultures were optimized using spinner flasks by closely monitoring cell growth, viability, and morphology. The results were used for scaling up the process to a ReadyToProcess…
Article
Flexible Facilities for Viral Vector Manufacturing
Another option would be to use adherent cells with microcarriers in stirred-tank bioreactors. Both options typically do not have scale limitations and thus would allow for a more scalable process.
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Article
Mapping a Route for Cell and Gene Therapy Process Development
While cell and gene therapies differ in many ways, some of the best practices for process development and validation are similar.
By Cynthia A. Challener
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Article
Scaling Up Novel Therapies
Scaling Up Novel Therapies
Platform processes have improved monoclonal antibody scale-up. Can they do the same for personalized therapies?
By Agnes S…