Search results for "filtration" in Articles / App Notes
Article
Downstream Processing for Cell-Based Therapies
Typically, a swing-bucket rotor centrifugation step or a filtration step is included to remove cell debris, large aggregates, unwanted proteins, volume reduce (i.e., concentrate), and formulate the ce…
Article
Updating Viral Clearance for New Biologic Modalities
Because viruses are typically smaller than downstream impurities such as host cell proteins (HCPs) and other extracellular debris, they require meticulous filtration, even in more established processe…
Article
Downstream Processing Continues to Worry CMOs
(Figure courtesy of author)
Chromatography and ultrafiltration
When it comes to specific steps that contribute to downstream-related capacity constraints, chromatography columns continue to be…
Article
Selecting a Comprehensive Bioburden Reduction Plan
Filtration
BioPharm: How do endemic bacteria affect the type of filter needed for filtration?
Mittelman and Anicetti (PDA task force): While bacteria (and fungi) may be present in all in-proce…
Article
Ensuring Viral Safety of Viral Vaccines and Vectors
Options include high temperature/short time (HTST), C spectrum ultraviolet light (UV-C), gamma irradiation, and nano- or virus filtration.
HTST is commonly used in the food and beverage industr…
Article
Selecting the Right Viral Clearance Technology
Ideally, methods from the first category, which include inactivation mechanisms such as low pH, detergent, or temperature hold steps and robust removal steps, such as virus filtration that clear a bro…
Article
Viral Clearance Challenges in Bioprocessing
In addition to treatment with heat and ultraviolet radiation, virus retentive filtration is recognized as one of the most efficient and robust virus removal steps, and implementation in the media feed…
Article
Challenges of Protein Aggregation During Purification
“All unit operations including chromatography, viral inactivation, viral filtration, and other filtration modes can create conditions that lead to protein aggregation, including shifts in pH and condu…
Article
QbD and PAT in Upstream and Downstream Processing
Pressure and filter volume represent CPPs for viral filtration steps. Again, if engineering controls permit tight control of these parameters, some may be downgraded to a lower parameter designation. …
Article
Concentration and Buffer Exchange Using the Automated, Single-Use ÄKTA™ readyflux System
Protein recovery was ≥ 99%, after 5‑times concentration and 5‑fold diafiltration. The process was performed according to set specifications for a tangential flow filtration (TFF) process, with the…