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Development beyond traditional monoclonal antibodies (mAbs) is increasing, and today, we see antibody variants like multispecific and bispecific antibodies and antibody fragments. In mAb processing, affinity chromatography is used to capture the target molecule based on specific interactions between the chromatography resin and the target molecule. For traditional mAbs, protein A chromatography is used to capture antibodies. Depending on the structure of the target antibody and the impurity profile, other affinities, like protein L or variants of protein A, may improve purity and increase removal of impurities.
Cytiva expands the antibody capture resin toolbox and has recently announced MabSelect™ VH3 affinity resin. MabSelect VH3 uses an engineered protein A resin that interacts only with the variable heavy chain containing the VH3 sequence family. This allows for efficient separation of bispecific antibody (bsAb) and antibody fragments such as Fab, scFv, and VHH that contain the VH3 sequence family.