Search results for " harvesting"
Article
Challenges in Cell Harvesting Prompt Enhancements
Cell harvesting involves the separation of the cell-culture from the medium and running it through several techniques: centrifugation, microfiltration, depth filtration, and filtration through absolut…
Article
Automated harvesting and 2-step purification of unclarified mammalian cell-culture broths containing antibodies
We report on a new integrated platform for automated harvesting of whole unclarified cell-culture broths, followed by in-line tandem affinity-capture, pH neutralization and size-exclusion chromatograp…
Article
Downstream Processing for Cell-Based Therapies
…emphasis on large-scale cell expansion; however, equally important is the development of large-scale harvesting and downstream processing operations. This paper presents and summarizes some of the ch…
Article
Combining Microbioreactors and Advanced Statistical Techniques to Optimize a Platform Process for a New Host-Cell Line
Use of a subspace model is a viable method to characterize process space variables and optimize process performance.
By Colin Jaques, Daniela Lega
Over the past decade, two improved capabili…
Article
Adherent Cell Culture in Biopharmaceutical Applications: The Cell-Detachment Challenge
While cell attachment may prove advantageous for some purposes, such as supernatant harvesting, cell detachment may become an enormous challenge when cells are to be harvested. Cell detachment is prob…
Article
Overview of a scale-up of a cell-based influenza virus production process using ReadyToProcess equipment
The aim of this white paper is to demonstrate how Cytiva single-use products can be applied in the field of vaccine manufacturing. The white paper includes a brief discussion around modern vacci…
Article
Single-Use Technologies Prove Effective for Viral Vector Process Development
The benefits of single-use technologies for upstream viral-vector processes clearly outweigh their disadvantages.
Cynthia A. Challener
Upstream viral-vector processes can have sig…
Article
A Risk-Based Genetic Characterization Strategy for Recombinant CHO Cell Lines Used for Clinical and Commercial Applications
The authors describe the ways in which manufacturers can mitigate the risks related to the integrity of recombinant transgenes expressed in CHO cells.
By Luhong He, Christopher Frye
Abstract
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Article
Technology Innovations Improve Process Chromatography Performance
As technology matures, inefficiencies and process limitations in downstream process chromatography are improved.
By Feliza Mirasol
Selecting an appropriate separation technology fo…
Article
Gene Therapies Push Viral Vector Production
Gene Therapies Push Viral Vector Production
Viral vectors show promise as a delivery mechanism for gene therapy, but which virus types are commercially viable?
By Feliza Miraso…