Search results for " antibody"
Article
FDA Provides Guidance on Bispecific Antibody Development Programs
“This guidance focuses on a range of regulatory and scientific considerations for bispecific antibodies, but not on development of a particular bispecific antibody. Of note, many aspects of a bispecif…
Article
Defining Critical Quality Attributes for Monoclonal Antibody Therapeutic Products
…e liquid chromatography with electrospray mass spectrometry (RP-HPLC/ES-MS) analysis of a monoclonal antibody. All figures are courtesy of the authors.
Mass Spectrometry for Molecular Weight Me…
Article
Evolution of the Monoclonal Antibody Purification Platform
Traditional Monoclonal Antibody Platform
Figure 1 illustrates the traditional mAb platform that is commonly used (2,3). Typical steps include:
• Protein A chromatography for capture of…
Article
Antibody Fragments and Their Purification by Protein L Affinity Chromatography
A majority of such molecules are monoclonal antibodies; however, many new entities are antibody fragments. Due to their structural, physiological, and pharmacological properties, antibody fragments of…
Article
Concentrating Feed—an Applicable Approach to Improve Antibody Production
Cell counts, antibody titer, protein quality analysis, and qPCR array. Cell-culture samples were counted by a Vi-CELL (Beckman-Coulter) using the trypan blue exclusion method. The pH and metabolites i…
Article
Guide for selecting affinity resins for mAbs, bsAbs, and antibody fragments
Today, molecules such as bispecific antibody (bsAb) and antibody fragments such as Fab, scFv, and VHH are seen in labs all over the world. These target molecules need to be purified to reach required …
Article
eBook: Purification of Antibody Therapeutics - Approach for Diversified Pipeline
Today, we see multispecific and bispecific antibodies, antibody fragments, and antibody drug conjugates in the development pipeline. Manufacturing platform approaches conveniently used for many tradit…
Article
USP Publishes Monoclonal Antibody Guidelines
USP standards for mAbs
Currently, licensed monoclonal antibody (mAb) therapeutics include those involved in the activation of effector cells, cell killing, cross-linked induced apoptosis, antagonis…
Article
Chromatography innovations for the diversified antibody pipeline
Our MabSelect™ VH3 affinity resin interacts with the variable heavy chain of the VH3 sequence family of human antibody, delivering high binding capacity and efficient separation of product-related imp…
Article
Optimization of a two-step purification method for bispecific antibodies
New and improved antibody bioprocesses and recombination techniques have contributed to a surge in the numbers of bispecific antibody constructs currently in clinical trials.
Alligator Bioscience A…