Search results for "column"
Article
Continuous Chromatography: Trends, Definitions and Approaches
All of the acronyms describe the same thing: a chromatography capture step-based utilizing multiple columns operated in a merry-go-round manner, where columns moved against the direction of feed flow.…
Article
Development of Purification for Challenging Fc-Fusion Proteins
In this study, utilizing a combination of high-throughput condition screening in 96-well plates, optimization in small-scale column models, and a novel virus-spiking strategy, the authors have develop…
Article
Unifying Continuous Biomanufacturing Operations
Downstream continuous purification has seen some success as well, mostly through the integration of various chromatography column sequences. But the continuous production of biologics in a closed, tot…
Article
Continuous Manufacturing: A Changing Processing Paradigm
They also note that continuous capture typically requires sterile chromatography columns. “For downstream polishing steps, the infancy of multi-column chromatography systems is a limitation,” they add…
Article
Analyzing Proteins Using SEC, MALS, and UHPLC
…molecular standards is not effective unless the proteins under study have similar conformations and column interactions as those of the standards. The combination of SEC with downstream light scatter…
Article
Evaluating the Use of Continuous Chromatography
…tion step is connected to an upstream perfusion or fed-batch bioreactor, and, by having two or more columns in the loading zone, you can overload the first column [because] any product/target molecul…
Article
Optimizing Cation-Exchange Chromatography with High-Throughput Process Development for mAb Purification
An example illustrating the use of HTPD and DoE to optimize the first polishing step for a mAb of interest is discussed herein, and the roles of both HTPD 96-well filter plates and mini-columns are hi…
Article
HT Multi-Product Liquid Chromatography for Characterization of Monoclonal Antibodies
CIEF usually is performed on commercial capillary electrophoresis instruments, which have a 20–60 cm long capillary and on-column ultraviolet (UV) absorbance detector. Imaged CIEF, or iCIEF, which use…
Article
Reducing Cross-Contamination Risks in Process Chromatography
Preparatory and post-processing steps such as column packing, validation of packed-bed efficiency, verification of endotoxin clearance, unpacking, cleaning of unpacked columns, etc., can also be elimi…
Article
Improving Process-Scale Chromatography
Capacity in a capture step such as Protein A is critical in reducing cost and increasing the ease of use through reductions in column size, buffer usage, and overall footprint of the unit operation,” …