Search results for "column" in Articles / App Notes
Article
The New World of Biopharmaceutical Manufacturing
Chromatography columns can be disposable or can be replaced by single-use membrane adsorbers. In the past couple of years, single-use centrifuges have been introduced. These have application as cell r…
Article
Asked and Answered: Your Questions on Ion-Exchange Chromatography
The questions are organized in by chief concern, and include buffers, experimental design, LC system and configuration, resin and column selection, specific applications, as well as troubleshooting an…
Article
Speed and Flexibility Are Dual Goals for Biopharma Operations
Other product introductions included new chromatography columns, inline process monitoring sensors, lab and pilot-scale filtration systems, and pumps and fluid handling systems. Suppliers also introdu…
Article
The Tide Stays High
There have even been suggestions that a venture capitalist might be appointed to run the agency (no appointments had been announced at the time this column was written). However, emerging bio/pharma i…
Article
Safety Drives Innovation in Animal-Component-Free Cell-Culture Media Technology
Resins, columns, tubing, connectors, filters, gaskets, mixing blades, and other process contact materials or ingredients are included in this scope. The producers of these items also need to understan…
Article
Single-use technologies in downstream process intensification
In bioprocessing, thorough cleaning is critical in order to take full benefit of the resin by removing impurities. When using conventional chromatography solutions, cleaning and cleaning validation …
Article
A Platform Approach for the Identity Testing of Multi-Component Cell-Culture Media
… (UHPLC) system from Waters Acquity equipped with photodiode array detector (PDA) and Acquity UHPLC column, BEH C18, 1.7 µm, 2.1 x 100 mm. A gradient separation was performed using mobile phase A: 50…
Article
Antibody Fragments and Their Purification by Protein L Affinity Chromatography
In the case of recombinant full-length antibodies with suitable Fc regions, two or three column purification processes centered around Protein A affinity chromatography have proven to be fast, efficie…
Article
Best Practices in Qualification of Single-Use Systems
The author discusses the current best practices in technical qualification of single-use systems.
By Weibing Ding, PhD
Single-use technology has been around for approximately two decades if …
Article
Handbook: Size Exclusion Chromatography Principles and Methods
The first step towards a successful separation is to select the correct medium and this handbook focuses on the most up-to-date SEC media and prepacked columns.
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