Search results for "column" in Articles / App Notes
Article
Discovering the unknown: How does in silico process development change PD scientists’ work?
A lot of the effects of the thermodynamics that are happening inside the chromatography column are scale independent. Therefore, it's really easy to also predict what happens at a larger or smaller sc…
Article
Report from the 6th International HTPD Conference
Two challenges highlighted were the current practice of using microscale columns since the flow rate is limiting for viral clearance in short columns, and that the precision in the models is not there…
Article
Digitalization: The Route to Biopharma 4.0
In two-column capture chromatography, dynamic control can be obtained using adaptive model-based control strategy that led to process yield of more than 85%. The data from high performance liquid chro…
Article
Updating Viral Clearance for New Biologic Modalities
…ein solutions using single pass tangential flow filtration (SPTFF) before loading them onto a resin column, such as Eshmuno Q resin column (MilliporeSigma), improves the productivity of the chromatog…
Article
Strategizing for Rapid Changeovers in Biologics Manufacturing
Stoll adds that some product-dedicated equipment must also be installed, in particular chromatography columns with packed resin for drug substance purification. “All these activities may potentially b…
Article
A Look at the Affinity Chromatography Landscape
Affinity chromatography for DNA-based biologics
Methacrylate monolithic columns have been an enabling tool for developing fast and efficient separation processes for DNA-based biologics with low …
Article
Extractables Studies for Single Use Systems Used in ADC Manufacturing, Part 1
To address those concerns, extractables studies were
performed on disposable chromatography column housings and disposable flow paths provided by Cytiva. The extractables studies were performed wi…
Article
Technology solutions simplifying chromatography unit operations
Chromatography operations typically involve steps such as resin slurry preparation, column packing, cleaning, unpacking, and resin storage. While each of the aforementioned steps can potentially be …
Article
Analysis of Glycosylation in Biosimilars
The interaction of the glycans with the column matrix depends on the overall structure of the glycans, not just their mass.
The various antennary structures give different retention times, and thu…
Article
Maximize resin usage with continuous bioprocessing
The technology employs three or more chromatography columns to create a continuous purification step. In a PCC setup, columns are switched between the loading and nonloading steps, such as wash and el…