Search results for " fed-batch"
Article
Being Thorough When Transferring Technology
In the case of microbial fermentation, heating and mass transfer demands can often be controlled by the fed-batch feed rate. Thus, you want to use a feed profile at the bench or pilot scale that will …
Article
Cost Considerations Drive Lean Technology in Biopharmaceutical Manufacturing
Traditional manufacturing processes for biologics make use of large, fed-batch reactors and oversized chromatography columns that end up wasting time or material. Alternatively, upstream process scale…
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Single-use Bioreactors Have Reached the Big Time
“Today, a continuous process can last significantly longer than current fed-batch processes, which means that there are higher expectations and a need for a high-level of sensing analytics, providing …
Article
Modeling Bioreactor Performance
Model effectiveness is determined by the quality and composition of the data inputs.
By Cynthia A. Challener
Bioreactor design, including the geometry of the tank and impeller and the locati…
Article
Designing a Biomanufacturing Facility Incorporating Single-Use Technologies
Figure is courtesy of Cytiva
Starting with upstream, the status of the cell line and whether the process should be batch, fed-batch, or perfusion needs to be decided. Details about the nature of…
Article
Tools for Continuous Bioprocessing Development
Nearly all of these microbioreactors are capable of both batch and fed-batch operation. For the needs of conventional biomanufacturing, these cultivation modes are sufficient. However, continuous cult…
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Optimization of Protein Expression in Escherichia Coli
Much higher cell densities may be obtained in fed-batch cultivations.
Another type of medium, the auto-induction medium, eliminates the need for the addition of an inducer, specifically for lactose…
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ReadyToProcess WAVE 25 Bioreactor System
The single-use ReadyToProcess WAVE 25 bioreactor system is a reliable and intuitive cell culture device for working volumes up to 25 L.
The system is based on a well-known rocking technology tha…
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Addressing the Challenges in Downstream Processing Today and Tomorrow
Cell culture was performed using numerous methods, including roller bottles and stirred tank bioreactors operated in perfusion, batch re-feed, or fed-batch mode (1).
Starting in 1986 with the lic…