Search results for " Downstream PD" in Articles / App Notes
Article
Efficient purification of meningococcal polysaccharides in a two-step chromatography workflow
This application note demonstrates the purification of meningococcal capsular polysaccharides using chromatography. The described process constitutes a viable alternative to the traditional proce…
Article
Protein Characterization Using SEC: Three Webinar Takeaways
The possibility to separate molecules by size under native conditions is powerful in protein analysis. With more than thirty years in protein science, Åke Danielsson, Research Director at Cyti…
Article
Your Protein Purification Questions Discussed with Cytiva R&D Scientists
Within ÄKTA club, web chats are held. In the web chats, ÄKTA system users are offered the opportunity to discuss their questions--related to lab-scale protein purification and ÄKTA sy…
Article
Efficient Purification of the Pertussis Antigens Toxin, Filamentous Haemagglutinin, and Pertactin in Chromatography Workflows
This application note describes the purification of pertussis toxin (PT), filamentous haemagglutinin (FHA), and pertactin (PRN) in a process based on modern chromatography resins. The described meth…
Article
Efficient Purification of Pneumococcal Polysaccharides in a Chromatography Workflow
This application note demonstrates the purification of pneumococcal capsular polysaccharides using chromatography. The described process constitutes a viable alternative to the traditional process f…
Article
Walk Away and Do More Screening Preps
Developing a process or looking for a clone of interest often requires screening large numbers of samples. Many analyses require purified protein, and substantial hands-on time might be allocated to…
Article
Development of column packing methods based on pressure flow measurements
Scale-up of a chromatography process might appear as simply increasing the column diameter to accommodate for the higher quantity of material to be processed, while maintaining the same bed height …
Article
A Platform Approach to Purification of Antibody Fragments
Antibody fragments constitute a promising class of biopharmaceutical products. As compared with full length antibodies, their fragments have unique properties that make them favorable for certain th…
Article
Overcoming Buffer Challenges With In-Line Conditioning
Preparation and storage of the large number of buffers required for biopharmaceutical production can be a challenge, particularly in large production scale. In this application note, we show how in-…
Article
Simplify chromatography method creation
Method creation is an essential part of preparing for automated protein purification runs. To save time and effort, method creation can be simplified and you can get to the point where you can start…