Search results for " binding"
Article
Development and Future of Protein A Chromatography Technology: Q&A with Jonathan Royce, Business Leader Chromatography Resins, Cytiva
In the last decade, significant development has also been made in the area of Protein A, both in term of binding capacity and stability, as discussed in a previous Q&A session. What do you feel are th…
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N-Glycan Analysis of Biotherapeutic Proteins
Interaction of the Fc region of IgG with Fc-γ receptors (FcγR) influences immune effector functions including antibody-dependent cell-mediated cytotoxicity (ADCC) by binding to Fcγ RIIIa, a mechanism …
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Optimization of Protein Expression in Escherichia Coli
The lacUV5 promoter, a derivative of the lac promoter, contains two mutations in the -10 region and an additional mutation at -66 within the catabolite gene activator protein (CAP) binding site. These…
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Comparing Protein A Resins for Monoclonal Antibody Purification
…id (HCCF), and vendors of mAb purification technologies must develop chromatography resins with high binding capacity to meet the demand. In addition, more cost-efficient cleaning procedures are nece…
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Elucidating Biosimilars Characterization
The extent and the exact structure of carbohydrates attached to a protein may affect its binding to its target receptor, its clearance from the body, and potentially, its immunogenicity. The manufactu…
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Next Generation Process Chromatography
Other convective flow adsorbers have existed in bioprocessing for many years but struggle in different aspects of the separation such as capture binding capacity and dilution of the target product, wh…
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Impurity Testing of Biologic Drug Products
“Other host cell proteins and binding agents carried over from purification columns may mimic the action of the therapeutic protein in assays, leading to mis-formulation of the product outside the the…
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Cost Considerations Drive Lean Technology in Biopharmaceutical Manufacturing
Under EMA regulations,
in-vitro studies must show that the antibody binds to the target antigen, that it is binding to representative isoforms of the three Fc gamma receptors, and that its Fab and…
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Protein L affinity resin is ready to move to manufacturing
The MabSelect™ VL protein L resin now offers improved alkaline stability compared to Capto™ L resin and a two-fold or more increase in dynamic binding capacity (DBC) in certain applications. After com…
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Innovative Chromatography Resins Can Improve Purity and Quality
… plasmids, these molecules may not be able to enter the pores of the beads, resulting in overall low binding capacities,” he points out. However, a new Fibro format (1) (from Cytiva) has shown to be …