Search results for "mRNA"
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Navigating Molecular Diversity
Are you working with biomolecules beyond mAbs?
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Scaling Up an E. coli Upstream Process for Plasmid DNA Production — from 2.5 to 160 L scale Fermenters
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This article summarizes the results of Escherichia coli growth performance for plasmid production in a 5 L glass fermentor, which was scaled up to 80 and 160 L scale in Xcellerex™ X…
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Strategizing for Rapid Changeovers in Biologics Manufacturing
The same goes for the mRNA platform, where processes are similar between different candidates. By using multiple platforms, the risk for failure can be mitigated,” he explains.
In addition, given …
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Gene Therapies Push Viral Vector Production
That being said, other technologies such mRNAs delivered to the target cells with, for example, mammalian cell-line produced engineered exosomes, start to show their capabilities,” Touw interjects. “A…
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Emerging Therapies Test Existing Bioanalytical Methods
It is also used to measure the expression levels of transgene- expressed mRNA in target tissue for gene therapy programs. PCR is a widely used platform; however, there is a lack of specific regulatory…
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Platform Technologies Improve Protein Expression
A cytomegalovirus (CMV) promoter drives the transcription of one mRNA from which two proteins are translated (i.e., the protein of interest [product] and a functionally impaired Zeocin selection marke…
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Patenting Prospects for Cell-Based Therapies
Myriad Genetics (“Myriad”) (8), in which the Court deemed patent ineligible DNA isolated from nature (while at the same time deciding that cDNA, made from mRNA converted by the viral enzyme reverse tr…
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Optimization of Protein Expression in Escherichia Coli
Depending on the protein target, the ability to tune the level of expression of the target mRNA may give certain advantages, such as increased protein accumulation, increased protein solubility, and i…
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Concentrating Feed—an Applicable Approach to Improve Antibody Production
In a qPCR array, 3 × 106 cells of day 9 and day 11 from each treatment were used to extract messenger ribonucleic acid (mRNA) following the protocol of RNeasy Mini Kit (Qiagen). 400 ng of total RNA fo…