Search results for " methods"
Article
How to Optimize Protein Desalting
Most protein purification workflows require desalting, buffer exchange, or both at one or more steps. But how do you determine which method to use? And how do you choose the right product for your n…
Article
Managing Risk in Raw Material Sourcing
To identify and assess risk, the company uses a documented risk-assessment process, facility audits, and a toolkit that includes such methods as failure modes and effect analysis, fishbone diagrams, a…
Article
Efficient Purification of the Pertussis Antigens Toxin, Filamentous Haemagglutinin, and Pertactin in Chromatography Workflows
This application note describes the purification of pertussis toxin (PT), filamentous haemagglutinin (FHA), and pertactin (PRN) in a process based on modern chromatography resins. The described meth…
Article
Improving Process-Scale Chromatography
Advances in technology are increasing the productivity and efficiency of commercial-scale chromatography bioprocesses.
By Cynthia Challener, PhD
Chromatography is a crucial step in the purif…
Article
A Platform Approach to Purification of Antibody Fragments
Antibody fragments constitute a promising class of biopharmaceutical products. As compared with full length antibodies, their fragments have unique properties that make them favorable for certain th…
Poster
Guide to modern BioProcess™ chromatography resins
Proteins are different. Based on what protein you would like to purify and the conditions used, chromatography resins are selected to optimize purity and yield. Many bioprocess applications include …
Article
Efforts Accelerate to Streamline Postapproval Change Process
…ease batch size, shift to a new manufacturing facility, or improve process robustness and analytical methods.
Expanded global sourcing and sales complicates lifecycle product management, though, …
Article
Perfusion in the 21st Century
Perfusion support
One of the challenges faced by all companies that are exploring use of perfusion processes is the limited ability to use high-throughput methods in perfusion mode. Currently, the…
Poster
Columns up to 1600 mm in diameter packed with protein A chromatography medium using axial mechanical compression
Different methods have been developed to pack a broad range of chromatography media (resins) in columns with axial mechanical compression. This includes packing of traditional BioProcess™ media, such …
Poster
Residual DNA analysis in influenza vaccine processing
In cell-based influenza vaccine production, the European Pharmacopoeia demands a host cell residual DNA concentration of less than 10 ng per dose. To reliably measure residual DNA in both process sa…