Search results for " affinity"
Article
Applications of Surface Plasmon Resonance for Detection of Bispecific Antibody Activity
…nt analysis) or by comparing and even fitting entire binding curves for determination of kinetic and affinity parameters. SPR systems can deliver a direct binding assay focused on relevant interactio…
Article
Optimizing Cation-Exchange Chromatography with High-Throughput Process Development for mAb Purification
…cells, and the initial mAb capture step was carried out with an alkali-stabilized, Protein A-derived affinity medium using a standard protocol. An HTPD approach was then taken for the subsequent puri…
Article
A Bright Future for the Plasma Fractionation Industry
Expanded bed adsorption (EBA) technology, precipitate protein isolation by membrane electrophoresis (PrIME+), and the affinity cascade (PPPS) all demonstrate progress in different parts or in differen…
Article
Evolution of the Monoclonal Antibody Purification Platform
The authors discuss the evolution of the purification platform for manufacturing of mAb therapeutics.
Monoclonal antibodies (mAb) increasingly form the majority share of the product pipeline…
Article
Report from the Eighth International Plasma Product Biotechnology Meeting Melia Salinas, Lanzarote, Spain
Report from the Lanzarote meeting May 13th – 17th, 2013
Yet another well-attended and successful Plasma Product Biotechnology (PPB) meeting has been held. The meeting focu…
Article
The Development and Application of a Monoclonal Antibody Purification Platform
Using this process, the pH of the Protein A affinity chromatography eluate was adjusted upward after pH inactivation and loaded directly onto the anion exchange resin. The anion exchange flow-through …
Article
QbD and PAT in Upstream and Downstream Processing
Rathore (IIT Delhi): For upstream process, tools that researchers have used include:
•Surface plasmon resonance (to assess product concentration and affinity)
•High-performance liquid chromato…
Article
Overview of a scale-up of a cell-based influenza virus production process using ReadyToProcess equipment
The multimodal function of the chromatography medium, utilizing both size exclusion and affinity binding, reduces the number of purification steps needed by combining two procedures in one. The proper…