Search results for " affinity"
Article
A Platform Approach to Purification of Antibody Fragments
However, with recent developments of novel affinity chromatography resins, there are new emerging possibilities. Here, we present affinity chromatography resins that offer the possibility of a platfor…
Article
Resolving a bottleneck in screening and characterization of recombinant antibody fragments using Biacore 4000
This method provides rapid screening in combination with data-rich selection of fragments with high affinity and the desired kinetic behavior. Kinetic analysis at different temperatures provided addit…
Article
Tackling Analytical Method Development for ADCs
Bioanalysis in biological fluids
From an immunoassay perspective, the development and validation of methods for the bioanalysis of ADCs require the consistent availability of high affinity, high s…
Article
SEC in the Modern Downstream Purification Process
Biomolecules typically should not bind to an SEC resin like they do in ion-exchange chromatography, affinity chromatography, hydrophobic interaction, or mixed-mode chromatography. As a result, mobile-…
Article
Ligand-Binding Assays and the Determination of Biosimilarity
In addition, ligand-binding assays enable the characterization of the potency of different biosimilars and provide crucial information on the affinity (dissociation rate constant, KD) and selectivity …
Article
Optimizing Resin Performance with Disposable Chromatography Solutions
Shawn Knol/GETTY IMAGES
By: Randi Hernandez
BioPharm International, spoke to Fredrik Lundström, product manager, Cytiva; Jonathan Royce, bioprocess senior product manager of antibody affinity …
Article
Defining Critical Quality Attributes for Monoclonal Antibody Therapeutic Products
To study the effects of glycan structure on the clearance of an Fc receptor fusion protein, an affinity extraction method was developed that could specifically recover the drug from cynomolgus monkey …
Article
Addressing the Challenges in Downstream Processing Today and Tomorrow
Feasibility has been demonstrated for a tandem downstream process for the purification of mAbs employing an affinity Protein A capture step followed by a flow-through anion-exchange (AEX) step and a v…
Article
Quality by design for biotechnology products—part 1
…proved stability; recombinant and chemical fusion approaches for improved half-life and potency; and affinity maturation by phage display or transgenic mouse (e.g., xenomouse, Humax, and velocity mou…
Article
Introducing the VH3 ligand ELISA kit
Accurate testing for ligand leakage from affinity resins is a key part of this process, ensuring compliance with regulatory standards.
Our new VH3 ligand ELISA kit - the latest addition to Cytiva…