Search results for " affinity"
Article
Optimize Column Loading Strategy to Gain Productivity in Protein Purification
Have you ever applied the concept of variable loading to gain productivity? By varying residence time (RT) over the loading phase, productivity from an affinity chromatography step can be significantl…
Article
Trends in Downstream Bioprocessing
It involves the removal of insoluble cell debris and particulates; product isolation through extraction, adsorption, ultrafiltration, or precipitation; product purification through affinity chromatogr…
Article
Get Started: Purification of mAb Variants - eLearning
The success of monoclonal antibodies (mAbs) as therapeutics and the multi-domain structure of antibodies has made it possible to create antibody variants, such as bispecific antibodies (bsAbs)…
Webcast
Cytiva Protein Select Technology Live Webinar
During a simple affinity step, the protein self-cleaves from the tag and elutes with no residual tag amino acids. The protocol does not require the use of a protease, and the same buffer can be used f…
Article
A deep dive into optimizing AAV capture and polishing to maximize both yield and purity
The affinity capture step maximizes recovery and efficiently removes impurities.
Join this webinar to:
• Learn how to optimize affinity capture
• Discover critical parameters that enhance f…
Article
Technology Innovations Improve Process Chromatography Performance
Specific affinity resins are also being developed that enable an effective and straightforward capture step that results in high purity. We can see this trend in new biological targets (e.g., adeno-as…
Article
Addressing the Complex Nature of Downstream Processing with QbD
“For instance, protein A or affinity resins in general, as well as flow through steps, display these properties since these steps in most cases build on a fundamental understanding of the physiochemic…
Article
Development and Future of Protein A Chromatography Technology: Q&A with Jonathan Royce, Business Leader Chromatography Resins, Cytiva
What is your response to these concerns?
It is true that Protein A affinity chromatography resins have higher cost per liter compared to, for example, ion exchangers or HIC resins. It is also tru…
Article
Improvements in Protein A Chromatography
Do you expect a similar approach with affinity resins and purification platforms to be used in these cases a swell?
Some molecules, like those having Fab domains, are amendable to the use of pla…
Article
Protein Purification Troubleshooting Guide
Practical advice to significantly impact your chromatography workflow
Put this poster on your lab wall to quickly identify and resolve protein purification problems when and where they happen. I…