Search results for " Fab"
Article
N-Glycan Analysis of Biotherapeutic Proteins
α-gal has been associated with IgE-mediated hypersensitivity reactions to Erbitux (cetuximab) produced in the mouse myeloma line SP2/0, where there are α-gal N-glycans on the Fab region (22). CHO cell…
Article
Cost Considerations Drive Lean Technology in Biopharmaceutical Manufacturing
The Fab region of an antibody interacts with the target, while the Fc is engaged in cell-mediated cytotoxicity. Under EMA regulations,
in-vitro studies must show that the antibody binds to the tar…
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Resolving a bottleneck in screening and characterization of recombinant antibody fragments using Biacore 4000
Chimeric chicken/human Fab fragments were screened and characterized using a preoptimized Human Fab Capture Kit to achieve accurate off-rate determination during initial screening of crude samples. Th…
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Top Tips for Successful Development of Antibody Chromatography Processes
We see more fragments, such as Fabs, scFv fusion proteins, domain antibodies, single chain fragments variable. And we also see bi- and multi-specific antibodies where you have variable site that can t…
Article
Get Started: Purification of mAb Variants - eLearning
The success of monoclonal antibodies (mAbs) as therapeutics and the multi-domain structure of antibodies has made it possible to create antibody variants, such as bispecific antibodies (bsAbs)…
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Chromatography processes for the diversified therapeutic antibody pipeline
The diversity of antibody variants in today’s pipeline presents unique challenges for purification and we see molecules such as bsAbs, Fabs, dAbs, and others. Conventional chromatography protocols, …
Article
As the therapeutic pipeline diversifies, chromatography toolboxes are expanding.
Development beyond traditional monoclonal antibodies (mAbs) is increasing, and today, we see antibody variants like multispecific and bispecific antibodies and antibody fragments. In mAb proc…
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Antibody purification quiz
Test your knowledge and gain insights in the purification of antibody variants – bsAbs, Fab, dAbs.
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Get to know: mAb variants - eLearning
The diversity of antibody variants in the biotherapeutics pipeline are increasing with a growing interest for molecules like antibody fragments and bispecific antibodies (bsAbs). Cytiva has pu…
Article
Platform for Purification of VHH-type Antibody Fragments
However, it is known that protein A can also bind to VH3 sequences located on the variable heavy chain of the Fab region.
This poster shows examples of purification of mAb-derived fragments.