Search results for " expression" in Articles / App Notes
Article
Fifteen Years of Progress: Biopharmaceutical Industry Survey Results
Trends in adoption of diverse mammalian expression systems have also not materialized, as Chinese hamster ovary (CHO) cell lines have become the dominant expressions systems. In fact, nearly 80% of re…
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Techniques for Improving Process Development for Exosome-Derived Therapeutics
One cancer treatment with enormous potential is for exosomes to deliver siRNA and knock down the expression of oncogenic mRNA. The laboratory of Dr. Raghu Kalluri, one of our founders at The Universit…
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The New World of Biopharmaceutical Manufacturing
The number of drugs that are produced by non-mammalian expression systems, such as yeast and microbes, will continue to climb; these will require more highly engineered microbial bioreactors.
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Witnessing Major Growth in Next-Generation Antibodies
He also notes that Fc-engineering can lead to mitigation of the body’s natural checkpoint mechanisms, which modulate the expression of T cells, resulting in amplification of cytolytic effects.
“Th…
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Perfusion in the 21st Century
Once the selection pressure is removed, those cell lines tend to lose expression (9). The use of a glutamine synthetase (GS) system maintains the selection pressure having to add an agent.
Importa…
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Affinity chromatography Handbook: Vol. 2 Tagged Proteins
The use of recombinant proteins has increased greatly in recent years, as has the wealth of techniques and products used for their expression and purification. The advantages of using a protein/peptid…
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A Platform Approach for the Identity Testing of Multi-Component Cell-Culture Media
Proteins are produced by gene expression in bacterial or mammalian cell culture. Cell-culture media are composed of essential raw materials that are required for cell growth and expression. Similar ce…
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Re-use of Protein A Resin: Fouling and Economics
These resins have been designed to keep pace with increasing bioreactor volumes and cell-culture expression levels and thereby prevent Protein A capture from becoming a bottleneck in the purification …
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Novel Vaccine Technologies Meet the Need for Pandemic and Therapeutic Solutions
New approaches to vaccine production are targeting rapid supply for pandemic situations and broadly effective therapeutic treatments.
Following the 2009 outbreak of the H1N1 pandem…
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Comparing Protein A Resins for Monoclonal Antibody Purification
Today, a typical process accumulates titers of 1-5 g/L, but expression levels as high as 10-13 g/L have been reported (5). The increase in upstream productivity creates a subsequent demand on downstre…