Search results for "host"
Article
Antibody Production in Microbial Hosts
Mammalian cells have been primarily applied as the hosts of choice for mAb expression. However, clinical success of mAbs has tremendously increased their demand, and this in turn has fueled interest i…
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Host cell protein quantitation using ELISA
Host cell proteins (HCPs) produced by cells during biologic manufacturing must be removed from the final product to protect patients. However, it’s challenging to detect HCP, and this step c…
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Challenging Host Cell Protein Assays for Improved Risk Mitigation
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This webinar shares Cytiva’s strategy for designing a comprehensive host cell protein (HCP) risk mitigation strategy. We will address how to challenge your assays ea…
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Current Trends in Host Cell Protein Detection for Biologics Manufacturing
Commonly accepted HCP analytical methods, the challenges of HCP assay, and recommendations on what can easily be accomplished in-house and when it may be better to outsource.
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Host-Cell Protein Measurement and Control
HCPs are proteins produced or encoded by the host organisms used to produce recombinant therapeutic proteins (1). Recombinant therapeutic proteins are usually produced by genetically-modified prokaryo…
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Impurity Testing of Biologic Drug Products
Some impurities (especially host cell proteins) may add an immune-stimulating or adjuvant behavior to the therapeutic, causing the patient to generate antibodies or cell-mediated immunity against the …
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Quality by design for biotechnology products—part 1
Examples of process-related impurities include host cell DNA, host cell protein, adventitious agents, residual medium supplements such as protein hydrolysates, and components such as nucleases or resi…
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Gene Therapies Push Viral Vector Production
Lentiviral vectors have displaced traditional retroviral vectors because lentiviral vectors do not require dividing host cells for integration, Carstens further explains. This characteristic broadens …
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Overview of a scale-up of a cell-based influenza virus production process using ReadyToProcess equipment
Impurities originating from the host cells were removed during the downstream process. Cell debris was removed in the first microfiltration step using ULTA Prime GF 2 μm and 0.6 μm normal flow filters…
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Platform Approach Speeds Process Development
…h as recombinant monoclonal antibodies is dependent on high performing bioprocesses using mammalian host cells. Chinese hamster ovary (CHO)-based cell culture platforms should combine state-of the-ar…