Search results for " bsAb" in Articles / App Notes
Article
Buffer optimization for separation of bsAb in a capture step using MabSelect™ VL resin
This video presents a study of the effects of different buffer substances, mono-, di-, and tri-carboxylic acids, and concentrations on elution pH, resolution between hetero- and homodimers for bsAb’s,…
Article
Guide for selecting affinity resins for mAbs, bsAbs, and antibody fragments
Development of antibody-derived molecules are increasing. Today, molecules such as bispecific antibody (bsAb) and antibody fragments such as Fab, scFv, and VHH are seen in labs all over the w…
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Exploring the world of antibodies: mAbs, Fabs, dAbs, and bsAbs
There is a vast array of antibody variants, including multispecific (msAb), bispecific antibodies (bsAb), antibody fragments (Fab, dAb, scFv), and antibody-drug conjugates (ADC). But with this…
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Optimizing KiH bispecific antibody chromatography steps
This ability allows bsAbs to be more effective at treating diseases. However, due to their increased complexity bsAbs often have higher levels of impurities in comparison to monoclonal antibodies (mAb…
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Single-Step, Protein A Chromatography Process for Bispecific Antibodies in Early Screening
The goal was to separate BsAb monomer from fragments such as the individual heavy and light chain pairs while achieving high purity and yield of BsAb. MabSelect™ PrismA protein A resin was selected fo…
Article
Selecting Chromatography Resins for Bispecific Antibody Purification
Their purification can be more challenging than traditional monoclonal antibodies (mAbs), however, due to the increased level of product-related impurities with high similarity to the desired bsAbs. A…
Article
Get Started: Purification of mAb Variants - eLearning
Purification of bsAbs and antibody fragments isn’t as straightforward as for mAbs. These molecules show wider variation in binding affinities and are often more challenging to separate from impurities…
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Optimization of a two-step purification method for bispecific antibodies
Bispecific antibodies (bsAbs) have gained increasing interest over recent years because they can bind two antigens, offering unique modes of action compared to monoclonal antibodies. New and i…
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Get to know: mAb variants - eLearning
The diversity of antibody variants in the biotherapeutics pipeline are increasing with a growing interest for molecules like antibody fragments and bispecific antibodies (bsAbs). Cytiva has pu…
Article
Optimization of a Two-Step Purification Method for Bispecific Antibodies
Alligator Bioscience AB develops antibody-based pharmaceuticals for cancer treatment, specializing in mono- and bispecific antibodies for tumor-directed immunotherapies. Alligator and Cytiva devel…