Search results for " NaOH"
Article
Cleaning Protein A Resins with 1 M NaOH
In this application note, resistance to high concentrations of NaOH is evaluated in protein A chromatography resins.
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Article
Evaluating E&L Studies for Single-Use Systems
• Many artifacts and analytical interferences were generated by strong/reactive solutions (as NaOH/NaClO), causing difficulties in the analyses and data interpretation; the use of strong saline/reacti…
Article
Concentrating Feed—an Applicable Approach to Improve Antibody Production
10 N NaOH was used to raise the pH until the solution was clear. After mixing well, the solution was made up to the required volume and filtered through a 0.22 µm filter (Millipore). The pH and osmola…
Article
Comparing Protein A Resins for Monoclonal Antibody Purification
…e conditions prevents the use of rigorous and cost-effective CIP and sanitization protocols based on NaOH.
Compared to conventional Protein A resins, one of the affinity chromatography resins inv…
Article
Alkaline stability of Fc and VH3 binding in protein A resins
We evaluated NaOH incubation and the effect of performed CIP cycles for two established protein A resins on the market.
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Article
Efficient Sodium Hydroxide Sanitization with a Novel Procedure of AxiChrom™ Columns Packed with Chromatography Resin
Here, we describe a microbial challenge test with a concentration of 1-M NaOH to mimic typical sanitization practices employed by the biopharma industry for a broad range of resins. The final version …
Article
Sanitization Opportunities for Protein A Resins
Here, we describe an approach to safely sanitize our new generation bioprocess protein A affinity resin for antibody purification—MabSelect PrismA—with 30 mM PAA combined with NaOH cleaning-in-place r…
Article
Sanitization of ÄKTA™ pilot 600 Using Sodium Hydroxide
Sanitization of the SNAP-connectors using 1 M NaOH was also successfully conducted.
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Article
Development and Future of Protein A Chromatography Technology: Q&A with Jonathan Royce, Business Leader Chromatography Resins, Cytiva
Now, 1M NaOH can in fact be used, improving the bioburden control, and the binding capacity is now up to 80 g/L. With an increased binding capacity and new high-flow agarose bead design, the productiv…
Article
Sanitization of AxiChrom™ Columns Packed with Chromatography Resin
This study evaluates sanitization methods based on 1 M sodium hydroxide (NaOH) as sanitization agent using methodology previously developed for peracetic acid (PAA).
Access study data here…