Search results for "Protein A" in Articles / App Notes
Article
Improving Process-Scale Chromatography
The development of new resin chemistries including enhancements to Protein A for monoclonal antibody (mAb) capture, increasing use of automation, implementation of single-use technologies, and explora…
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Process Chromatography: Continuous Optimization
…bal product marketing manager for Cytiva, are typically purified using a platform approach in which protein A chromatography resins are used in the first capture step. Polishing is then achieved usin…
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Antibody Production in Microbial Hosts
coli, the Sec-dependent pathway has been used for protein secretion because of its abundance, although accumulation of protein aggregates may occur due to the generation of premature proteins before s…
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Cell-free Expression Systems Pose Cell Culture Alternative
Cell-free expression is promising in preclinical applications, but still presents challenges to scale up for commercial production.
Feliza Mirasol
Among the challenges in upstrea…
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Advances in Engineering of Protein-Based APIs
“Protein therapeutics traditionally were designed as single receptor/protein antagonists or agonists. But to effectively combat disease, many future drugs will require multiple proteins being agonized…
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Concentrating Feed—an Applicable Approach to Improve Antibody Production
Antibody titer was measured using high-performance liquid chromatography (HPLC) (Waters Corporation) equipped with a protein A column (Applied Biosystems). The protein charge profile was analyzed by i…
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Innovative Chromatography Resins Can Improve Purity and Quality
A generic capture step for mAbs and Fc fusion proteins has been robustly established by classical Protein A affinity resins, says Oliver Schub, director of Business Development at Navigo Proteins, a s…
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Continuous Manufacturing: A Changing Processing Paradigm
To standardize downstream operations, he says one option would be “the rational design of highly specific ligands for the capture step analogous to Protein A in mAb purification” (3).
Costs of ado…
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Reimagining Affordable Biosimilars
With new configurations and solid-state designs, SPR has become a workhorse in protein and nucleic acid applications. The sensor surfaces have been modified to allow cell-based binding to mimic real-t…
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Process Chromatography Selection for Downstream Processing Applications
In the case of Fc-fusion proteins, Protein A can still be utilized as a capture step.
Levison (Pall): [Some commonly used separation technologies include] ion-exchange, mixed-mode, hydrophobic int…