Search results for "Protein A" in Articles / App Notes
Article
Production of recombinant proteins using a novel affinity technology
This scientific paper from Journal of Chromatography A describes results of a study utilizing a Cytiva Protein Select™ affinity resin and tag platform to produce any recombinant proteins.
Re…
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How To Interpret Protein Binding Capacity: Chromatography Resins
The comparison between protein binding capacities of resin from different suppliers is not always straightforward. Different methods may have to be used, and often these methods are not stated direc…
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Multistep Protein Purification: Gain Time and Consistency
In this webinar, "Gain Time and Add Consistency with Automated Multistep Protein Purification," the presenters discussed the benefits of automation, how to set up an automated purification protocol,…
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Your Protein Purification Questions Discussed
In one of Cytiva´s web chats, ÄKTA™ system users asked questions relating to lab-scale protein purification and ÄKTA systems, which experts answered during a live session.
The expert…
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Work from Anywhere and Add Flexibility to Protein Purification Workflows
The protein purifier of today has a full agenda and many different tasks to perform. It can be difficult to find time for attending full runs in the lab. And when there is time, it might be a challe…
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Insights from process developers - top challenges in recombinant protein purification
You might be one of the many process developers who are working on a diversity of molecules such as enzymes, peptides, scaffolds, and other recombinant proteins. This constantly evolving molecul…
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Alkaline stability of Fc and VH3 binding in protein A resins
In this study, we have looked at how different antibody binding sites of a protein A ligand are affected by cleaning in place (CIP). We evaluated NaOH incubation and the effect of performed CIP cycles…
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Purifying Tagged Proteins: Learn the Basics
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What is tagged protein purification? When and why should you use it? Learn the basics in our quick guide to tagged protein purification.
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What If You Could Remove Your Antibody Aggregates Already in the Protein A Step?
…es: (1) elution buffer type, (2) conductivity of the elution buffer, (3) pH gradient length and (4) Protein A resin type.
Article
Rapid Cycling Protein A-Based Fiber Chromatography to Address Emerging Downstream Bottlenecks
The protein A fiber matrix has an open pore structure in which mass transfer is governed by convective flow. This structure allows high monoclonal antibody (mAb) binding capacities at very short resid…