Search results for " analyze"
Article
Tips for Purifying and Analyzing Cryo-EM Samples
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Cryogenic electron microscopy (cryo-EM) provides possibilities to determine structures for more challenging proteins, such as membrane proteins and larger protein compl…
Article
Analyzing Proteins Using SEC, MALS, and UHPLC
Light scattering analysis combined with more rapid size exclusion chromatography improves protein characterization.
The characterization of proteins involves identification of their complex …
Article
Virus-like Particles as Therapeutic Moieties of the Future
Mass spectrometry (MS) is a robust tool to analyze the molecular mass, amino acid composition, and post-translational modifications (PTMs) of the capsid proteins present in VLPs. The high sensitivity …
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A Platform Approach for the Identity Testing of Multi-Component Cell-Culture Media
glucose concentration (mg/mL) was analyzed by linear regression.
Analysis of culture media—The SPF culture media was analyzed as part of system suitability (described in the following passages). S…
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HT Multi-Product Liquid Chromatography for Characterization of Monoclonal Antibodies
Typically, protein samples are submitted, prepared, and analyzed, and then results are reviewed and reported. When developing high throughput or multi-product analytical methods, it is important to ke…
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Assessing Manufacturing Process Robustness
All data sets prepared, transposed, and analyzed should be subject to data verification procedures to assure data accuracy and integrity.
Documents required for the assessment include the…
Article
Modeling the Degradation of mAb Therapeutics
The samples were analyzed for aggregation content using size-exclusion high performance liquid chromatography (SE–HPLC) and types of oligomers using dynamic light scattering (DLS) (7) (Figure 1A).
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N-Glycan Analysis of Biotherapeutic Proteins
This “top-down” approach requires minimal sample preparation, although reduction can be used to analyze heavy and light IgG chains separately, or can be fragmented with specific proteases, such as Ide…
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Implications of Cell Culture Conditions on Protein Glycosylation
A permethylation derivatization procedure is then performed on the separated N-glycans and O-glycans, enabling them to be analyzed using matrix-assisted laser desorption ionization–mass spectrometry (…
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Improving PAT for Biologics
Unique challenges
The key to successfully implementing PAT for bioprocesses is the availability of robust, reliable, low-cost, easy-to-use online/atline analyzers; a complete understanding of the va…