Search results for " plasmids" in Articles / App Notes
Article
Scaling Up an E. coli Upstream Process for Plasmid DNA Production — from 2.5 to 160 L scale Fermenters
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This article summarizes the results of Escherichia coli growth performance for plasmid production in a 5 L glass fermentor, which was scaled up to 80 and 160 L scale in Xcellerex™ X…
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Improving Capacity in DNA Plasmid Purification
Working with plasmids? Plasmid supply is in high demand these days. Join Tony Hitchcock on May 6, 2020 to learn more about this critical market and how Cobra Biologics is working with Cytiva …
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Modern plasmid purification process
AcuraBio selects Cytiva's latest two-step purification protocol to offer a productive plasmid DNA service to its customers globally to alleviate supply constraints for mRNA and cell & gene th…
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Purification of Plasmid DNA
Plasmid DNA is the building block of advanced therapies. As these therapies take off, so does manufacturing demand. By switching to modern chromatography resins, Cobra Biologics was able to increase…
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A scalable single-use two-step plasmid purification process
The current global demand for biopharmaceuticals is over USD 300 billion, with an estimated growth of ≥ 12% annually. While MAb processes have become much more efficient, production of mRN…
Article
Akron Bio Will Install a Cytiva FlexFactory to Manufacture Plasmid DNA
Cytiva will provide the first FlexFactory to be used in the manufacture of pDNA to Akron Bio.
Cytiva will provide its FlexFactory, a flexible, single-use platform, to enable Akron Biotech to m…
Article
A Risk-Based Genetic Characterization Strategy for Recombinant CHO Cell Lines Used for Clinical and Commercial Applications
The authors describe the ways in which manufacturers can mitigate the risks related to the integrity of recombinant transgenes expressed in CHO cells.
By Luhong He, Christopher Frye
Abstract
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Article
Gene Therapies Push Viral Vector Production
“Apart from plasmids there are liposomes, lipoplexes, polyplexes, and oligonucleotides [that can be used] as non-viral modes of gene delivery.”
Vicaire also notes that using non-viral vehicles …
Article
Optimization of Protein Expression in Escherichia Coli
…e efficient repression, the plasmid must also carry the lacI or lacIq gene, especially for high copy plasmids. The lacIq gene contains a mutation in its promoter that enhances lacI expression by tenf…
Article
Scalable Viral Vector Manufacturing
…ciated Virus, the virus can be produced upon introduction into the host cell line by transfection of plasmids encoding both the viral genes and the gene of interest. Thus, the plasmids are used as a …