Search results for " protein"
Article
Cleaning Protein A Resins with 1 M NaOH
If not removed, these impurities can build up on the protein A resin and start to leach into the process material in subsequent cycles. The presence of cell culture nutrients in the harvest feed loade…
Article
Affinity chromatography Handbook: Vol. 2 Tagged Proteins
The use of recombinant proteins has increased greatly in recent years, as has the wealth of techniques and products used for their expression and purification. The advantages of using a protein/peptid…
Article
Handbook: Design of Experiments in Protein Production and Purification
In a protein research lab or during process development, a DoE approach can greatly improve the ef ciency in screening for suitable experimental conditions, for example, for cell culture, protein sepa…
Article
Antibody Fragments and Their Purification by Protein L Affinity Chromatography
In the case of recombinant full-length antibodies with suitable Fc regions, two or three column purification processes centered around Protein A affinity chromatography have proven to be fast, efficie…
Article
Protein L affinity resin is ready to move to manufacturing
The MabSelect™ VL protein L resin now offers improved alkaline stability compared to Capto™ L resin and a two-fold or more increase in dynamic binding capacity (DBC) in certain applications. After com…
Resource
Protein A Fiber Chromatography from Research to Scale-up and Manufacturing of Monoclonal Antibodies
This makes the pre-packed, small-scale Protein A fiber unit a versatile tool for processes such as clone selection and upstream cell-culture media development. The time savings in downstream processes…
Article
Capacity and Performance of a Next Generation Protein A Chromatography Resin
Click here to read application note: Capacity and performance of MabSelect™ PrismA protein A chromatography resin
For more information, visit MabSelect Protein A resins
Article
High Throughput Protein Purification at a Glance
Frida Henningson Johnson, Annelie Cajander, and Anne-Sophie Svensson share their knowledge about high-throughput protein purification with readers in this blog.
Poster
A Next Generation Protein A Chromatography Resin for Improved Productivity and Bioburden Control
• The increased upstream titers in mAb production seen in recent years may make the Protein A capture step a bottleneck.
• Protein A columns columns are also more prone to bioburden contamination…
Article
Protein Purification Questions and Answers from ÄKTA Club Web Chat
Or do you need support to get on with your protein purification? Around 40 protein purifiers joined our latest ÄKTA club web chat to get answers to their questions. Web chats, where Cytiva expert…