Search results for " protein" in Articles / App Notes
Article
Cleaning Protein A Resins with 1 M NaOH
If not removed, these impurities can build up on the protein A resin and start to leach into the process material in subsequent cycles. The presence of cell culture nutrients in the harvest feed loade…
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Affinity chromatography Handbook: Vol. 2 Tagged Proteins
The use of recombinant proteins has increased greatly in recent years, as has the wealth of techniques and products used for their expression and purification. The advantages of using a protein/peptid…
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Handbook: Design of Experiments in Protein Production and Purification
In a protein research lab or during process development, a DoE approach can greatly improve the ef ciency in screening for suitable experimental conditions, for example, for cell culture, protein sepa…
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Antibody Fragments and Their Purification by Protein L Affinity Chromatography
In the case of recombinant full-length antibodies with suitable Fc regions, two or three column purification processes centered around Protein A affinity chromatography have proven to be fast, efficie…
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Protein L affinity resin is ready to move to manufacturing
The MabSelect™ VL protein L resin now offers improved alkaline stability compared to Capto™ L resin and a two-fold or more increase in dynamic binding capacity (DBC) in certain applications. After com…
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Capacity and Performance of a Next Generation Protein A Chromatography Resin
Click here to read application note: Capacity and performance of MabSelect™ PrismA protein A chromatography resin
For more information, visit MabSelect Protein A resins
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High Throughput Protein Purification at a Glance
Frida Henningson Johnson, Annelie Cajander, and Anne-Sophie Svensson share their knowledge about high-throughput protein purification with readers in this blog.
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Protein Purification Questions and Answers from ÄKTA Club Web Chat
Or do you need support to get on with your protein purification? Around 40 protein purifiers joined our latest ÄKTA club web chat to get answers to their questions. Web chats, where Cytiva expert…
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High Throughput Protein Purification at a Glance
The project uses antibodies to study the localization of proteins in human tissues and cells. In a blog post site manager Hanna Tegel and research engineers Anne-Sophie Svensson and Annelie Cajander, …
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Process development using a nonaffinity method vs Cytiva™ Protein Select™ resin
The first method is using nonaffinity chromatography steps (AIEX + heat treatment + HIC), while the second method is using the novel affinity Cytiva™ Protein Select™ resin for the capture step, follow…