Search results for " protein" in Articles / App Notes
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Production of recombinant proteins using a novel affinity technology
This scientific paper from Journal of Chromatography A describes results of a study utilizing a Cytiva Protein Select™ affinity resin and tag platform to produce any recombinant proteins.
Re…
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How To Interpret Protein Binding Capacity: Chromatography Resins
Explore what protein binding capacity is, how it can be measured and what exactly to look for on a manufacturer’s specification sheet in this blog post.
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Multistep Protein Purification: Gain Time and Consistency
During the webinar, questions were asked about different aspects of multistep protein chromatography and how to automate multiple protein purification steps. A summary of the benefits and gains using …
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Your Protein Purification Questions Discussed
Questions about chromatography techniques, troubleshooting tips and tricks, and the use of ÄKTA systems were submitted in writing by protein purifiers globally.
We have included the questio…
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Work from Anywhere and Add Flexibility to Protein Purification Workflows
Does this sound familiar?
Did you know that you can free up time and work remotely with your protein purification system?
ÄKTA™ chromatography systems and UNICORN™ software featu…
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Insights from process developers - top challenges in recombinant protein purification
In this article, we focus on the challenges in purifying recombinant proteins that lack affinity chromatography solutions.
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Alkaline stability of Fc and VH3 binding in protein A resins
In this study, we have looked at how different antibody binding sites of a protein A ligand are affected by cleaning in place (CIP). We evaluated NaOH incubation and the effect of performed CIP cycles…
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Purifying Tagged Proteins: Learn the Basics
When and why should you use it? Learn the basics in our quick guide to tagged protein purification.
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What If You Could Remove Your Antibody Aggregates Already in the Protein A Step?
…ies: (1) elution buffer type, (2) conductivity of the elution buffer, (3) pH gradient length and (4) Protein A resin type.
Article
Rapid Cycling Protein A-Based Fiber Chromatography to Address Emerging Downstream Bottlenecks
The protein A fiber matrix has an open pore structure in which mass transfer is governed by convective flow. This structure allows high monoclonal antibody (mAb) binding capacities at very short resid…